YCGP Proteomics and Mass Spectrometry Facility

Sample submission guidelines

Please do not prepare, run gels or send samples without first discussing the project and prep procedures with our director, Eugene Davidov. Optimum protocols can change frequently---one of the most significant determinants of the success of your analysis will be the preparation.

Please make copies of the attached forms and save your originals.

Enclosed is a form for the services offered at the YCGP Proteomics Facility.

Each sample must be accompanied by a completed form. The information you provide on these forms helps to ensure proper handling of your sample. Please include both a PTAEO or purchase order number and the purchase order amount---samples submitted with incomplete billing information (i.e., no PO number) cannot be accepted. Analysis of your sample will be delayed until complete billing information is received.

If you are submitting a sample for protein identification, only the DIGESTION/SEPARATION FORM should be completed. We track the subsequent LC/MS/MS and/or sequencing process from this form. You should not send a MASS SPEC or PROTEIN SEQUENCE FORM. The latter are only used for individual MW determinations or intact N-terminal sequence determinations, respectively.

When requesting an amino acid analysis to quantitate your sample for N-terminal sequencing, you should include with your PROTEIN SEQUENCE ANALYSIS FORM(S) a completed AMINO ACID ANALYSIS
FORM specifying the % for AAA and the % for sequence.

Important information to include on your forms:

• Sample Name (which should match the name on the sample tube).
• Your phone number and fax number.
• Your billing information: PO number, billing address, and urchasing agent phone number; please arrange to send a hard copy of your PO when ready, either with your sample or by fax.
• The MW, quantitation (even if only an estimate), and method of quantitation.
• The organism or species (not the vector, please) from which your polypeptide is derived.

Note: Radiolabelled samples can not be submitted for analysis at this time.

For non-Yale users it is best to send samples via FedEx; if you choose to submit samples in person please be advised that drop-off hours are Monday through Friday, 10:00 AM to 3:00 PM.

REMEMBER: All fields in the submitted forms must be completed!
INCOMPLETE or INCORRECT FORMS WILL DELAY YOUR SAMPLE!

Eugene J. Davidov, Ph.D., Director
Yale University, 219 Prospect St, KBT, 700, New Haven, CT 06520
eugene.davidov@yale.edu, 203-432-4709